Macromolecular Size-and-Shape Distributions by Sedimentation Velocity Analytical Ultracentrifugation
نویسندگان
چکیده
منابع مشابه
Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.
Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particles, determined from the experimental data, which scales the diffusion coefficie...
متن کاملSedimentation Velocity Analytical Ultracentrifugation
(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a sing...
متن کاملAnalytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
متن کاملStudying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation
Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation. Title Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation. Publication Type Journal Article Year of Publication 2005 Authors Balbo, A, Minor, KH, Velikovsky, CA, Mariuzza, RA, Peterson, CB, Schuck, P Journal Proc Natl Acad Sci U S A Volume 102 Is...
متن کاملCharacterisation of chitosan-mucin complexes by sedimentation velocity analytical ultracentrifugation
There is increasing interest being expressed by the pharmaceutical industry in how chitosan molecules interact with the mucus and mucosal epithelia lining the gasterointestinal and tracheobronchial systems (Fiebrig et al., 1995a). Interest in their use for drug delivery is principally because of their polycationic properties, biodegradability and lack of antigenicity. Three routes have been rec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2006
ISSN: 0006-3495
DOI: 10.1529/biophysj.106.081372